PYY stimulates synthesis and secretion of intestinal apolipoprotein AIV without affecting mRNA expression.

We tested whether exogenous peptide YY (PYY) can stimulate synthesis and lymphatic secretion of intestinal apolipoprotein AIV (apo AIV). Rats with mesenteric lymph fistulas and right atrial cannulas were given continuous intravenous infusions of control vehicle or PYY at 25, 50, 75, 100, or 200 pmol ⋅ kg-1 ⋅ h-1. PYY (75-200 pmol ⋅ kg-1 ⋅ h-1) stimulated lymphatic apo AIV output from 1.5- to 3.5-fold higher than basal output. In separate experiments, PYY (100 pmol ⋅ kg-1 ⋅ h-1) produced a 60% increase in jejunal mucosal apo AIV synthesis but had no effect on mucosal apo AIV mRNA levels at doses up to 200 pmol ⋅ kg-1 ⋅ h-1. Finally, exogenous PYY infusion (100 pmol ⋅ kg-1 ⋅ h-1) produced a plasma PYY increment of 30 pM compared with an increment of 18.7 pM in response to ileal infusion of lipid. These results support the hypothesis that PYY may be an endocrine mediator of the effects of distal gut lipid on production and release of intestinal apo AIV, likely via a posttranscriptional mechanism of action.